EuroMRD Publications

Filter tags:
RQ-PCR
Guidelines
Ph+ ALL
ddPCR
NGS
RNAseq
Methods Mol Biol book on Immunogenetics

One-Step Next-Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

Villarese P, Abdo C, Bertrand M, Thonier F, Giraud M, Salson M, Macintyre E

Reference: Methods Mol Biol. 2022;2453:43-59

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_3

Methods Mol Biol book on Immunogenetics
NGS

Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

van der Velden VHJ, Bastian L, Brüggemann M, Hartmann AM, Darzentas N

Reference: Methods Mol Biol. 2022;2453:61-77

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_4

Methods Mol Biol book on Immunogenetics
RNAseq

Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR

Starza ID, Eckert C, Drandi D, Cazzaniga G; EuroMRD Consortium

Reference: Methods Mol Biol. 2022;2453:79-89

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_5

Methods Mol Biol book on Immunogenetics
ddPCR

Quality Control for IG /TR Marker Identification and MRD Analysis

Fronkova E, Svaton M, Trka J

Reference: Methods Mol Biol. 2022;2453:91-99

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_6

NGS
Methods Mol Biol book on Immunogenetics

cfDNA-Based NGS IG Analysis in Lymphoma

Pott C, Kotrova M, Darzentas N, Brüggemann M, Khouja M; EuroClonality-NGS Working Group

Reference: Methods Mol Biol. 2022;2453:101-117

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_7

Methods Mol Biol book on Immunogenetics
NGS

Targeted Locus Amplification as Marker Screening Approach to Detect Immunoglobulin (IG) Translocations in B-Cell Non-Hodgkin Lymphomas

Genuardi E, Alessandria B, Civita AM, Ferrero S

Reference: Methods Mol Biol. 2022;2453:119-132

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_8

NGS
Methods Mol Biol book on Immunogenetics

PCR Technology to Identify Minimal Residual Disease

Cazzaniga G, Songia S, Biondi A; EuroMRD Working Group

Reference: Methods Mol Biol 2021;2185:77-94

Link: https://pubmed.ncbi.nlm.nih.gov/33165844/

RQ-PCR

Potential and pitfalls of whole transcriptome-based immunogenetic marker identification in acute lymphoblastic leukemia; a EuroMRD and EuroClonality-NGS Working Group study

Van der Velden VHJ, Brüggemann M, Cazzaniga G, Scheijen B, Tops B, Trka J, Pal K, Hänzelmann S, Fazio G, Songia S, Langerak AW, Darzentas N; EuroMRD; EuroClonality-NGS Working Group

Reference: Leukemia 2021;35:924-928

Link: https://pubmed.ncbi.nlm.nih.gov/33608635/

NGS

Standardisation and consensus guidelines for minimal residual disease assessment in Philadelphia-positive acute lymphoblastic leukemia (Ph + ALL) by real-time quantitative reverse transcriptase PCR of e1a2 BCR-ABL1

Pfeifer H, Cazzaniga G, van der Velden VHJ, Cayuela JM, Schäfer B, Spinelli O, Akiki S, Avigad S, Bendit I, Borg K, Cavé H, Elia L, Reshmi SC, Gerrard G, Hayette S, Hermanson M, Juh A, Jurcek T, Chillón MC, Homburg C, Martinelli G, Kairisto V, Lange T, Lion T, Mueller MC, Pane F, Rai L, Damm-Welk C, Sacha T, Schnittger S, Touloumenidou T, Valerhaugen H, Vandenberghe P, Zuna J, Serve H, Herrmann E, Markovic S, Dongen JJMV, Ottmann OG

Reference: Leukemia 2019;33:1910-1922

Link: https://pubmed.ncbi.nlm.nih.gov/30858550/

Ph+ ALL
RQ-PCR

Analysis of minimal residual disease by Ig/TCR gene rearrangements: guidelines for interpretation of real-time quantitative PCR data

Van der Velden VHJ, Cazzaniga G, Schrauder A, Hancock J, Bader P, Panzer-Grumayer ER, Flohr T, Sutton R, Cave H, Madsen HO, Cayuela JM, Trka J, Eckert C, Foroni L, Zur Stadt U, Beldjord K, Raff T, van der Schoot CE, van Dongen JJM; European Study Group on MRD detection in ALL (ESG-MRD-ALL)

Reference: Leukemia 2007;21:604-11

Link: https://pubmed.ncbi.nlm.nih.gov/17287850/

Guidelines
RQ-PCR